Angelica anti-β -amyloid protein in nerve cells caused by toxic effect of.docVIP

 PAGE \* MERGEFORMAT 17 Angelica anti-β -amyloid protein in nerve cells caused by toxic effect of Author: Gu Ran, Liu jade, Zhang Lan Jiang, Xiao-Yan Hao, Xiao-Yan, Xiao-Lan Qi, Ke-Ren Shan, Xi-Lin Ren, Guan Zhi-zhong [Abstract] Objective To study the Angelica on β -amyloid protein induced SH-SY5Y neural cells, the nicotine receptor subunit to reduce the level of protein, cell injury and lipid peroxidation in antagonism to understand their possible mechanism of the neuroprotective effect. Methods certain concentration of Angelica pretreatment SH-SY5Y cells, then add β -amyloid protein processing, cells was determined by colorimetric MTT reduction rate, the overall level of understanding from the cells of Angelica on the cell activity; were measured by Western blotting neural nicotinic receptor subunits (α 3 and α 7) protein expression level changes; by TBA colorimetric assay of lipid peroxidation products (MDA) levels, in order to understand the antioxidant capacity of Angelica. The results of the safety levels of Angelica extracts decreased β -amyloid protein that causes cell damage and elevated levels of lipid peroxidation against β -amyloid protein that causes the α 3 and α 7 nicotinic receptor subunit protein levels lowered. Conclusion Angelica have some neuroprotective effect. [Keywords:] Angelica; SH-SY5Y cells; nicotine receptor; β -amyloid protein; lipid peroxidation Abstract: ObjectiveTo investigate the inhibitory effects of Angelica on the neurotoxicity and the decreased expression of nAChRs induced by b-amyloid peptide (Ab) in human neuroblastoma (SH-SY5Y) cells. MethodsThe SH-SY5Y cells were treated by a certain concentration of angelica , and then exposed to 10 ml Aβ 25-35. MTT reduction assay was carried out to detect the cell viability, lipid peroxidation was measured by TBARS to observe the level of oxidative stress, and the expression of nicotinic receptor α 3 or α 7 subunits at protein level was detected by Western blot. ResultsAngelica at