Angelica neonatal rats after intrauterine hypoxia on renal vascular endothelial growth factor expression.docVIP

 PAGE \* MERGEFORMAT 11 Angelica neonatal rats after intrauterine hypoxia on renal vascular endothelial growth factor expression [Abstract] Objective Angelica intrauterine hypoxia on neonatal rat kidney vascular endothelial growth factor (VEGF expression. Methods pregnancy 14 d of SD rats were randomly divided into control group, hypoxic group and the angelica group. hypoxia group, 14 pregnant rats from gestation day once daily by intravenous injection of normal saline 8 ml / kg (continuous 7d, after a low tension induced by hypoxia model of intrauterine hypoxia of embryo 2 h (row 3 d: 14,15,16 days of gestation, Angelica treatment group 250 g / L of Angelica injection instead of saline, the other dealing with the hypoxic group and control group was not lack of oxygen, the other group with the hypoxia group .3 date of delivery of pregnant mice of renal tissue, paraformaldehyde fixed, paraffin embedded sections, VEGF immunohistochemistry, 400 times the camera, IPP6.0 image analysis software. Results neonatal hypoxia group, the number of VEGF positive cells and integrated optical density values than the control group increased (P lt;0.05), Angelica VEGF-positive cells and the integrated optical density compared with hypoxia group decreased (P lt;0.05). Conclusion certain period of time, a certain oxygen concentration in neonatal rats Hypoxia can stimulate the kidneys increased expression of VEGF, and angelica injection can decrease the expression of VEGF. [Keywords:] Angelica, hypoxia, renal, vascular endothelial growth factor Vascular endothelial growth factor (vascular endothelial growth factor, VEGF) in regulating angiogenesis in the basic growth factor, abundant in the kidney, has been more evidence that VEGF in the kidneys play an important role in embryonic development. Intrauterine hypoxia affecting the embryonic development of common factors, and renal ischemia and hypoxia is one of the most sensitive organs, VEGF is the regulation of angiogenesi