Angelica Hung Qi membrane extract mixture ECV-304 cells on vascular endothelial growth factor mRNA expression.docVIP

 PAGE \* MERGEFORMAT 13 Angelica Hung Qi membrane extract mixture ECV-304 cells on vascular endothelial growth factor mRNA expression [Abstract] Objective Angelica Hedysari membrane extract mixture on human umbilical vein endothelial cells (ECV-304) of vascular endothelial growth factor (VEGF) mRNA expression. Methods cultured ECV-304 cells as a model, using tetrazolium assay (MTT) cell proliferation determined, semi-quantitative reverse transcription - polymerase chain reaction detection of ECV-304 cells the expression of VEGF mRNA. Results angelica mixture of red Qi membrane extract on ECV-304 The role of cell proliferation, compared with the control group significantly (P lt;0.05), angelica mixture of red Qi membrane extract can induce the expression of VEGF mRNA, whose role was positively correlated with the dose. Conclusion Angelica mixture Hedysari membrane extract on ECV-304 cell proliferation by inducing mechanism of ECV-304 cells to achieve expression of VEGF mRNA. [Keywords:] angelica, red Qi, endothelial cells, vascular endothelial growth factor Abstract: Objective To study the effect of the ultra-filtration extract from Angelica sinensis and Hedysarum polybotrys’s mixture on the VEGF mRNA expression of ECV-304 cell. Method Angelica sinensis and Hedysarum polybotrys’s mixture were refined by ultra-filtration technique. ECV-304 cells were cultured as model, and its proliferation was detected by MTT colorimetry. VEGF mRNA expression was observed by semi-quantitative RT-PCR. Results The ultra-filtration extract from Angelica sinensis and Hedysarum polybotrys’s mixture could markly promote the growth of ECV-304 cells , there was significant difference between experimental and control group (P lt;0.05). The ultra-filtration extract from Angelica sinensis and Hedysarum polybotrys’s mixture upregulated VEGF mRNA expression with dose-dependent relation. Conclusion The ultra-filtration extract from Angelica sinensis and Hedysarum polybotrys’s mixtu