Anisodamine treatment of traumatic acute lung injury in rabbits experimental study of molecular biological mechanism.docVIP

 PAGE \* MERGEFORMAT 2 Anisodamine treatment of traumatic acute lung injury in rabbits experimental study of molecular biological mechanism Author: Chien-wen, FAN Ai-lin, Yin, Qi Hao-Wen, Bai Changqing [Abstract] Objective: To investigate the anisodamine (654  2) pairs of rabbits with traumatic acute lung injury (ALI) the molecular mechanisms of therapeutic effect. Methods: traumatic acute lung injury model, rabbits were randomly divided into control Group (CG), the lung injury group (IG) and treatment group (EG), n = 24. animals in each group, respectively, after the establishment of lung injury model 1, 2, 3, 4 h of bloodletting killed six, specimens from bronchial alveolar lavage fluid (BALF), isolated alveolar macrophages (PAM), using gel electrophoresis mobility shift assay (EMSA) and RT  PCR to detect PAM in the NF  κ B activity changes and TNF  α mRNA expression in levels were detected by ELISA in BALF supernatant TNF  α and IL  8 content. Results: IG NF  κ B activity, TNF  α mRNA expression and TNF  α , IL  8 levels in the model 1 ~ 4 h after the establishment of were significantly higher than that in CG (P