Apigenin in vitro of mouse T lymphocyte proliferation cell cycle and apoptosis.docVIP

 PAGE \* MERGEFORMAT 15 Apigenin in vitro of mouse T lymphocyte proliferation cell cycle and apoptosis Of: end of District had Yao Ying, Huang Xiuyan, He Fang [Abstract] Objective: apigenin (AP proliferation of mouse T cells, the cell cycle and apoptosis. Methods: sterile isolated from mouse lymph nodes and thymus cells, MTT assay at different concentrations (25, 50, 100, 150, 200 mol / L of the AP of the polyclonal stimulant concanavalin A (ConA) induced proliferation of T cells, PI staining and flow cytometry (FCM to analyze cell cycle distribution, Annexin V-FITC/PI double AP staining and FCM detection of T cell apoptosis, and the AP with dexamethasone (DEX common role of changes in T cell apoptosis, and MTT assay using the drug concentration on the T cells in vitro. Result: 25 ~ 200 mol / L AP on the role of T cells without drug toxicity, and significantly inhibited ConA-induced T cell proliferation (P lt;0.01), the cell cycle arrest in G0/G1 phase, and dose-dependent; the concentration of T cells withered AP death were inhibited, and significantly inhibited the DEX-induced T cell apoptosis (P lt;0.01), and dose-dependent. Conclusion: In a certain range of concentration, AP significantly inhibited ConA-induced T cell proliferation in mice, so that cell cycle arrest in G0/G1 phase and inhibited T cell apoptosis. [Keywords:] apigenin apoptosis of T cell proliferation cycle [Abstract] AIM: To study the effect of Apigenin (AP) on the proliferation, cell cycle and apoptosis of mouse T cells in vitro. METHODS: The lymphocytes were prepared from lymph nodes and thymus of mice. The effect of AP on the proliferation of T in response to ConA at different concentrations (25, 50, 100, 150, 200 mol / L) was detected by MTT. Cell cycle was measured by PI staining and FCM. The effect of Apigenin and Apigenin with DEX on T cell apoptosis was measured by Annexin V-FITC/PI double staining and FCM. The effect of different concentrations of AP cytotoxicity to T cel