ApoE gene polymorphism hsCRP and coronary heart disease study.docVIP

 PAGE \* MERGEFORMAT 11 ApoE gene polymorphism hsCRP and coronary heart disease study [Abstract] Objective To analyze the apolipoprotein E (ApoE gene polymorphism, high-sensitivity C-reactive protein (hs CRP levels and coronary heart disease. Methods nucleic acids using phenol chloroform extraction method to separate from the clot, using polymerase chain reaction (PCR, Hha1 enzyme digestion in 94 CAD patients and 100 healthy controls were ApoE gene polymorphism was detected by automatic biochemical analyzer, in order to strengthen the immune latex turbidimetric assay of serum hs CRP content. Results ApoE allele frequencies: coronary heart disease compared with the control group, 2 allele frequency was significantly lower (P lt;0.01, 4 allele frequency increased (P lt;0.005, serum hs CRP levels, coronary heart disease significantly increased (P lt;0.05, but different alleles, hs CRP was no significant difference (Pgt; 0.05. Conclusions of the Joint Monitoring ApoE gene polymorphism and serum hs CRP levels on cardiovascular disease risk prediction sensitive and valuable indicator. [Keywords:] apolipoprotein E, gene polymorphism, high-sensitivity C-reactive protein, coronary heart disease [Abstract] Objective To analyze the polymorphism of apolipoprotein E (ApoE), and high sensitivity C reactive protein (hs CRP) levels and the relevance of coronary heart disease (CHD). Methods Phenol chloroform extraction was used to get the DNA from the isolated blocks blood coagulation. Polymerase chain reaction, Hhal endonuclease digestion were used to test the 94 cases of CHD patients and 100 healthy control group ApoE polymorphism. Hs CRP level was measured by immune latex transmission turbidity. Results ApoE allele frequency: compared with the control group, 2 allele frequency decreased significantly (P = 0.01) in CHD group, 4 allele frequency increased (P = 0.005). Serum hs CRP concentration in CHD group was significantly higher than that of control grooup (P l