Apoptin prokaryotic expression vector and expression in Escherichia coli.docVIP

 PAGE \* MERGEFORMAT 12 Apoptin prokaryotic expression vector and expression in Escherichia coli Of: Jian-Sheng Wang, Zhang Mingxin, a small section of art, Wang Zheng, Zhou Suna, Zhang Guangjian, Wangquan Ying, Yang Guang laugh [Abstract] Objective To construct Apoptin prokaryotic expression vector and preparation of antigens Apoptin fusion protein. Method to obtain Apoptin fusion gene on the basis of the successful construction of the Apoptin of prokaryotic expression vector pET-28a (+)-Apoptin, the plasmid transformed into E. coli E.coli BL21 (DE3) receptor bacteria to IPTG induction of its expression, polyacrylamide gel electrophoresis protein. Apoptin results into a prokaryotic expression vector pET-28a (+)-Apoptin E. coli E.coli BL21 (DE3) by IPTG induction, by SDSanalysis of the relative molecular mass of about 17 000 positions on the target protein bands, size, and Apoptin fusion protein. Conclusion Apoptin prokaryotic expression vector pET- 28a (+)-Apoptin fusion protein can be expressed Apoptin for further study and preparation of Apoptin antibody Apoptin basis. [Keywords:] Apoptin prokaryotic expression vector pET-28a (+) E. coli ABSTRACT: Objective To construct an Apoptin prokaryotic vector, aiming to produce antigenic fusion protein Apoptin. Methods The Apoptin gene was amplified from the template of plasmid pSSCHG/NT4-Apoptin- HA2-TAT by PCR. The Apoptin was sub-cloned into the multiple clone sites of plasmid pET-28a (+) to get the prokaryotic vector of pET-28a (+)-Apoptin, which was transformed into E.coli BL21 (DE3). Expression of E.coli BL21 (DE3) was induced by IPTG . The specific protein expression was detected by SDS. Results The fusion protein was expressed with high efficiency in E.coli BL21 (DE3) transformed by pET-28a (+)-Apoptin after induction with IPTG. The specific fusion protein had an apparent related molecular weight of about 17 000 ku as indicated by SDAanalysis. Conclusion The Apoptin prokaryotic expression vector