Application of DNA microarray technology arsenic trioxide on RPMI 8226 cells.docVIP

 PAGE \* MERGEFORMAT 14 Application of DNA microarray technology arsenic trioxide on RPMI 8226 cells Author: Wang Meng-chang, Liu SX, Liu boom [Keywords:] Arsenic trioxide [Abstract] Objective: Using cDNA microarray study of arsenic trioxide on multiple myeloma cell line RPMI 8226 role. Methods: contains 4096 human genes in cDNA microarray to detect the role of arsenic trioxide on RPMI 8226 cells before and after 24 h of their gene expression changes. Results: The mRNA level, 273 genes significant changes have taken place, in which increased expression of 121 genes, 152 genes were down-regulated. Conclusions: Arsenic trioxide can cause a series of RPMI 8226 cell line gene expression change. ZFYVE16, TXNIP, and ALK1 genes may be related to RPMI 8226 cell differentiation and apoptosis are closely linked. [Keywords:] Arsenic trioxide; DNA microarray; multiple myeloma Changes of gene expression profile of multiple myeloma cell line RPMI 8226 treated by arsenic trioxide ABSTRACT Objective: To compare the changes of gene expression profiles of multiple myeloma cell line RPMI 8226 before and after 24hour intervention of arsenic trioxide. Methods: The responses of the RPMI 8226 cells to arsenic trioxide were determined with cDNA microarray which included 4 096 different human genes. Results: Of these 4 096 genes, the expressions of 273 genes were altered significantly at mRNA level. The expressions of 121 genes were upregulated while the expressions of 152 genes were downregulated. Conclusion: The effect of arsenic trioxide on RPMI 8226 cells is related to changing the expression levels of a number of genes. ZFYVE16, ALK1 and TXNIP genes may play important roles in apoptosis and differentiation of RPMI 8226 cells. KEY WORDS arsenic trioxide; DNA microarray; multiple myeloma Multiple myeloma (multiple myeloma, MM) is a malignant proliferation of plasma cell disease. As the MM very low percentage of tumor cell proliferation and the formation of multi-drug resista