Arachniodes fern extracts against HIV-1 integration of the research activity.docVIP

 PAGE \* MERGEFORMAT 8 Arachniodes fern extracts against HIV-1 integration of the research activity Of: Li Huimin, Yin Chang-Chang, Wu Jiazhong, Zhang Lei [Abstract] Objective Arachniodes Dryopteridaceae Polystichum fern species in different leaf extracts in vitro anti-HIV-1 integration of research activity. Methods in vitro cell-free system by enzyme-linked immunosorbent assay overall HIV-1 and the half inhibitory concentration of enzyme (IC50). Results Arachniodes fern ethyl acetate extract, butanol extract inhibited HIV-1 integrase median effective concentration of IC50 were 44.30,16.02 μg / ml, water extract inhibited HIV-1 integrase median effective concentration of IC50gt; 100μg/ml. Conclusions Arachniodes ethyl acetate extract of fern and butanol extract of HIV-1 integration in vitro inhibitory activity. [Keywords:] Arachniodes fern extract; anti-HIV-I integration activity Abstract: ObjectiveTo evaluate the anti-HIV-1IN activities of the different extract parts from Arachniodes exilis Ching. MethodsHIV-1 IN reverse transcriptase, protease and integrase were detected by enzyme-linked inmunosorbent assay. ResultsIn the experiment of anti-HIV IN, the IC50 of the n-butanol extract acetic ether extract were 44.30, 16.02μg/ml, respectively. The IC50 of the aqueous extract was higher than 100 μg / ml.ConclusionThe n-butanol extract, acetic ether extract from Arachniodes exilis Ching have the inhibitory effect on HIV-1 IN activities. Keywords:: Arachniodes exilis; The different extracted parts; Anti-HIV-1 Arachniodes fern Arachniodes exilis Ching as Dryopteridaceae Arachniodes fern species, widely distributed in tropical and subtropical world regions to the east and southeast Asia, the most abundant species, mainly distributed in the Yangtze River in China south and Shandong and Henan provinces, with heat and dampness, anti-bacterial anti-inflammatory, sedative effect, civil for the treatment of acute jaundice hepatitis, arthritis, Yao Tuiteng,