Arctiin inhibitory effect on pancreatic cancer cells and its mechanism of experimental study.docVIP

 PAGE \* MERGEFORMAT 9 Arctiin inhibitory effect on pancreatic cancer cells and its mechanism of experimental study [Abstract] Objective To arctiin of pancreatic cancer cell line (CAPAN-1) in vivo experiments to verify whether they have the effect of anti-pancreatic cancer. Method in CAPAN-1 cell culture medium plus arctiin train 72 h and observed the proliferation of cancer cells and cancer cells inhibited the rate of morphological changes. By flow cytometry (FCM) detection of CAPAN-1 cell cycle and apoptosis rate; TUNEL assay CAPAN-1 cell apoptosis rate; immunocytochemical detection of CAPAN-1 cell proliferation, apoptosis regulatory protein (bax, bcl-2 ) expression. CAPAN-1 cells produced by Balb / c nude mouse tumor model, continuous daily intraperitoneal injection of arctiin (10 mg * kg-1 * d-1) 10d, with 5-FU (30 mg * kg-1 * d-1) as positive control observed tumor inhibition rate. Results arctiin inhibited CAPAN-1 cell growth; CAPAN-1 cell cycle arrest in G0/G1 phase and induce apoptosis; by upregulating the expression of bax protein (P amp;lt;0.01), reduced bcl-2 protein expression (P amp;lt;0.05 )-induced CAPAN-1 cell apoptosis; inhibit tumor-bearing nude mice, tumor growth inhibition rate was 41.7%. Conclusion arctiin with anti-pancreatic cancer activity, one of the mechanisms of its effect is to induce apoptosis. [Keywords:] arctiin; anti-tumor activity; apoptosis; CAPAN-1 cell line Abstract: ObjectiveTo evaluate the anti-tumor effect and mechanism of arctiin on the human pancreas cancer cell (CAPAN-1) in vitro and vivo. MethodsCAPAN-1 cell was cultured in medium containing serial concentration of arctiin for 72 hours. Then the growth rate and the morphological change were analyzed. Cell apoptosis percentage was measured by TUNEL method and FCM. Cell cycle phase distribution was measured by FCM. The expression of proliferation and apoptosis associated proteins of bax, bcl-2 in CAPAN-1 cell were determined using immunocytochemistry . CAPAN-1 cel