Ardisia mamillata acid dye colorimetry of total alkaloid content of.docVIP

 PAGE \* MERGEFORMAT 16 Ardisia mamillata acid dye colorimetry of total alkaloid content of 【Abstract】 Objective To determine the Ardisia mamillata method for the determination of total alkaloids. Solvent Extraction Methods acid dye colorimetry to dehydrogenase thistle base (C16H22N4O2) as the reference substance, bromocresol green as acid dyes, using chloroform extraction, detection wavelength 418 nm. Results dehydrogenase thistle Bases in 0.72 ~ 14.02 mg / L within the range of absorbance is linear regression equation was: Y = 19.254X-1.6247, r = 0.999 5 (n = 5), the average recovery was 101.65 %, RSD of 0.75% (n = 5). Conclusion The method is simple, accurate, and selectivity. Keywords: Ardisia mamillata total alkaloids; acid dye colorimetry; dehydrogenase Carduus base Abstract: ObjectiveTo establish a method for quantitative determination of the total alkaloids in the root of Ardisia mamillata.MethodsThe content was determined by solvent extraction and acid dye colorimetiy, with Acanthoine Ruscope-ine (C16H22N4O2) as a control criterion and bromocresol green as the acid dye, and the wavelength was at 418 nm. ResultsAcanthoine Ruscope-ine showed a good linear relationship with absorbance when its concentration was within 0.72 ~ 14.02 mg / L. The regression equation established was Y = 19.254X-1.624 7, r = 0.999 5 (n = 5). The recovery of Acanthoine Ruscope-ine was 101.65%, RSD was 0.75% (n = 5). ConclusionThis method is easy, accurate and specific. Keywords:: Ardisia mamillata total Alkaloid; Acid Dye Colorimetiy; Acanthoine Ruscope-ine Ardisia mamillata Ardisia mamillata, also known as red blankets, Baoding red, angel in red, Mao parasols, golden Red Bead, etc. for Myrsinaceae Ardisia. Country of Origin China, located in Fujian, Guangxi, Guangdong, Yunnan, Sichuan, Guizhou, Jiangxi and other places. Ardisia mamillata leaves purple, bright red fruit, leaves fruit and the United States, is the first choice for indoor f