Arsenic-resistant cell lines of human gallbladder carcinoma GBC SD - As the establishment of differential gene expression and apoptosis detection.docVIP

 PAGE \* MERGEFORMAT 12 Arsenic-resistant cell lines of human gallbladder carcinoma GBC SD / As the establishment of differential gene expression and apoptosis detection Of: Wuda Long Zhang Chengwen He Xiaowei [Abstract] Objective: To establish a resistance to arsenic trioxide (arsenic trioxide, As) in human gallbladder carcinoma cell line GBC SD / As, of GBC SD / As cell lines and the corresponding sensitive cell line GBC SD between differences in a variety of apoptosis-related genes investigate the expression of As-induced apoptosis in gallbladder cancer drug resistance associated with a variety of correlation between genes. Methods: As the concentration increased in vitro induction of human gallbladder carcinoma cell line GBC SD, establishment of resistant cell line GBC SD / As ; MTT method As cytotoxicity; use with 458 apoptosis related genes in human cell apoptosis-related gene cDNA microarray resistant cell lines the expression of apoptosis-related genes. Results: After 8 months induction training, successful As people build tolerance gallbladder cancer cell line GBC SD / As, compared with the GBC SD cells, and its resistance to the As a multiple of 13.6 times; gene chip test results showed that compared with the GBC SD cells, GBC SD / As in the 17 gene expression level of 2 times more significant changes, including increased expression of 6 genes, 11 genes were downregulated. Conclusion: 17 apoptosis related genes and GBC SD / As the resistance is closely related cells, suggesting that cells abnormal expression of apoptosis related genes may be the gallbladder As one of the molecular mechanisms of drug resistance. [Keywords:] gallbladder; resistance; apoptosis; arsenic trioxide Arsenic trioxide (arsenic trioxide, As) is the main component of Chinese medicine arsenic .20 80 century, the first report of scholars, As of acute promyelocytic leukemia have a significant effect, then, As quickly became a worldwide anti-cancer drug research hot [1].