Asian Taenia enolase gene and its protein structure and function of.docVIP

 PAGE \* MERGEFORMAT 25 Asian Taenia enolase gene and its protein structure and function of Abstract analysis and prediction of Asian Taenia enolase gene and its encoded protein structure and characteristics used to guide the experimental study of its biological function. 【Methods】 The U.S. National Center for Biotechnology Information and the Swiss Institute of Bioinformatics in protein analysis expert system on gene and protein sequence and structure information analysis tools, in combination with other bioinformatics analysis software package, such as Pcgene and Vector NTI suite, from the Asian tapeworm cDNA plasmid library to identify full-length enolase genes and their coding regions, analysis, prediction of the gene encoding the protein of the physical and chemical characteristics of the post-translational modification sites, functional domains, sub-cellular localization , topology, secondary structure, three-dimensional conformation and so on. 【Results】 The gene length 1737 bp, coding region for the first 205 ~ 1503 bp, encoding 433 amino acids, for full-length gene. GenBank with Echinostoma enolase amino acid sequence homology of the highest, reaching 78%. Theoretical predictive value of molecular weight 46653.5 Ku. No plastids, mitochondria targeting sequence. Predicted encoded protein has a transmembrane domains, three hydrophilic parts. With trematodes of the genus enolase recent evolutionary relationship. 【Conclusion】 The method of biological information from the Asian tapeworm adult cDNA library were screened out of the Asian tapeworm enolase full-length cDNA sequence and predicted by their structure and function of information. Keywords: Asian tapeworm enolase structure can bioinformatics Abstract: 【Objective】 To get the messages on the structures and characteristics of enolase from Taenia asiatica (T.a.ENO) by bioinformatics. 【Methods】 A full-length cDNA sequence encoding enolase from cDNA plasmid library of Taenia asiatic