Assessment of tissue microarray with colorectal cancer molecular margin preliminary study.docVIP

 PAGE \* MERGEFORMAT 13 Assessment of tissue microarray with colorectal cancer molecular margin preliminary study Of: Li Nan, Wang Jing United States, Ping-Ping Wu, Xu Jiajia, Jie Ding, Fang Yuan, Pei-Lin Huang [Abstract] Objective: To analyze the p53, Ki 67 in different cancer tissues and adjacent distance (1,2,3 cm from the tumor tissue differential expression in colorectal surgical margin of the molecular boundary. METHODS: The tissue microarray technique and immunohistochemistry of 44 cases of colorectal cancer and adjacent tissues of different distances p53, Ki 67 expression. Results: p53, Ki 67 in colorectal cancer and adjacent different distance (1,2,3 cm from the tumor tissues showed the trend of gradually weakened, and its statistical significance decreased expression. Conclusion: p53, Ki 67 trend shown by differential expression suggest that the surgical margins in colorectal cancer tissues in the molecular boundary. application of tissue microarray large-scale testing of clinical tissue samples, with a fast, convenient, economical and accurate. [Keywords:] colorectal cancer, tissue microarrays, molecular boundary, p53, Ki 67, surgical margin Colorectal cancer is the most common gastrointestinal malignant tumor, since its onset hiding, early often without obvious clinical manifestations, it is easy to delay in diagnosis and therapy, a serious threat to human health. Currently, surgical resection is the treatment of colorectal One of the most important means of cancer, and surgical treatment of local recurrence is a failure and an important prognostic reasons, the success of the procedure will influence the prognostic factors of colorectal cancer. the traditional method to determine whether a clean surgical cut is based on margin of normal tissue pathology (ie cell morphology, but recent studies show that tumor cell morphology changed significantly in the previous [1], its molecular and biochemical levels have abnormal metabolic changes of