Asthenospermia sperm mitochondrial DNA A3 243G G3 316A and T3 394C mutation.docVIP

 PAGE \* MERGEFORMAT 12 Asthenospermia sperm mitochondrial DNA A3 243G G3 316A and T3 394C mutation Of: Ling Xuemei Fang Ke, Lou Zhe Feng, Zhang Wei, Zhang Li Ya, Dragon King [Abstract] Objective: To detect weak sperm sperm mitochondrial tRNAleu 3 243 sites, sites of the mitochondrial ND1 3 316 and 3 394 point mutation frequency of the three mutation and the relevance of weak sperm. Methods: WHO standard of 69 cases were collected in patients with weak sperm samples and 60 normal sperm motility normal sperm samples by polymerase chain reaction, Apa and Hae restriction enzyme length polymorphism analysis and sequencing to detect mtDNA A3 243G, G3 316A and T3 394C mutations in three loci were compared between mutation frequency. analysis G3 316A and T3 394C mutation in combination with amino acid variation of bioinformatics tools to analyze the evolutionary conservation of missense mutations of amino acids. Results: weak sperm disorders of sperm mtDNA G3 316A mutation rate (4.35%, T3 394C mutation rate (2.90% and the total mutation rate (7.25% and the normal control group (5.00%, 1.67% and 6.67%, the difference was not significant. amino acid variation analysis found, G3 316A (Ala-gt; Thr, T3 394C (Tyr-gt; His are missense mutations, T3 394C mutation of the amino acid in the human, cow, mouse, Xenopus four kinds of highly evolved organisms conservative. Conclusion: G3 316A and T3 394C mutation may not affect human sperm motility the major factor. [Keywords:] mtDNA mutations asthenospermia Abstract: Objective: To detect the mutation frequencies at the locis of mtDNA 3 243,3316 and 3 394 in sperms and asthenospermia, to analyze the correlationship between the mutation asthenospermia at three loci. Methods: After 69 asthenospermia cases and 60 control cases were collected under the WHO criterion, the point mutations of mtDNA 3 243,3316 and 3 394 in sperms was detected using PCR-RFLP (polymerase chain reaction and restriction endonuclease fragment lengt