Azoospermia severe oligozoospermia patients with cytogenetic and Y chromosome microdeletions of.docVIP

 PAGE \* MERGEFORMAT 14 Azoospermia severe oligozoospermia patients with cytogenetic and Y chromosome microdeletions of Of: Yang Li, Peng Liang, Hu Xiaoping, Jiao Haiyan, the party clean, Chenyin Tao, Zhong Huijun, Lu Wang [Abstract] Objective azoospermia, oligozoospermia and severe oligozoospermia with chromosome abnormalities and Y chromosome azoospermia factor (AZF) microdeletions. Methods of peripheral blood lymphocytes by routine chromosome preparation methods and multiplex polymerase chain reaction technique, 136 cases (98 cases of azoospermia, oligospermia in 24 cases, 14 cases of severe oligozoospermia) in patients with male infertility cytogenetic and molecular genetic testing. The results of chromosome abnormality rate was 25% (34 / 136), which azoospermia 19.9% #8203;#8203;(27/136), oligozoospermia accounting for 2.2% (3 / 136), severe oligozoospermia total 2.9% (4 / 136), Y chromosome AZF deletion rate of 6.6% ( 9 / 136), which accounted for 5.1% azoospermia (7 / 136), severe oligozoospermia accounted for 1.5% (2 / 136). Conclusion chromosomal abnormalities and Y chromosome microdeletions in male infertility is a major genetic factor, Y chromosome anomaly and a certain relationship between AZF deletion. [Keywords:] azoospermia, severe oligozoospermia, chromosomal abnormalities, AZF microdeletions Abstract: Objective To investigate the relationship between chromosome abnormalities and Y chromosome microdeletion of azoospermia factor (AZF) in patients with azoospermia and oligozoospermia. Methods Both routine periphearl blood lymphocytes cytogenetic analysis and detection of AZF region microdeletion of Y chromosome by multiplex PCR were carried out in 136 infertile men, including 98 patients with azoospermia, 24 patients with oligozoospermia and 14 patients with sever oligozoospemia. Results Chromosomal abnormalities were found in 34 (25%) cases including 27 azoospermia, 3 oligozoospermia and 4 severe oligozoospermia. Microdeletions in AZF