Blood Xifeng with focal cerebral ischemia in rats endothelin-1 and nitric oxide content.docVIP

 PAGE \* MERGEFORMAT 13 Blood Xifeng with focal cerebral ischemia in rats endothelin-1 and nitric oxide content Of: Li Gao Hong, Liu Zhao pure, Wan Wen Ju, Song Xiaolan, Duan Rui, Su Xuehui [Abstract] Objective To observe the blood Xifeng with focal cerebral ischemia model in rat blood and brain tissue endothelin-1 (ET-1) and nitric oxide (NO) content, to explore the mechanism of brain protection . Methods Male Wistar 40 rats were divided into 5 groups: sham operation group, model group and blood Xifeng side of high, medium and low dose group, n = 8. using photochemical method to establish focal cerebral ischemia model, 7 d before modeling oral administration, blood Xifeng side of high, medium, low dose group were given blood Xifeng Fang 20,10,5 g / (kg · d), sham operation group and model group received volume of distilled water, 24 h after the model of blood and brain tissue were taken, rats were determined by radioimmunoassay in plasma and brain tissue content of ET-1, Determination of serum and brain tissue NO content. put out the results of blood rats can decrease wind plasma and brain tissue ET-1 content and decreased NO levels in brain tissue, increased serum NO levels compared with the model group, the difference was statistically significant (P lt;0.05 or P lt;0.01) . Conclusion blood Xifeng with focal cerebral ischemia model in rats may be related to decreased plasma and brain tissue ET-1 content and decreased NO levels in brain tissue, increased serum NO levels, which regulate ET-1 / NO ratio of about. [Keywords:] blood Xifeng Fang, endothelin 1, nitric oxide, cerebral ischemia, Rat Abstract: Objective To observe the effects of Huoxue Xifeng Decoction (HXD) on endothelin-1 (ET-1) and nitric oxide (NO) levels in the blood and brain tissue of focal cerebral infarction (FCI) rats. Methods Forty male Wistar rats were randomized into 5 groups: sham-operation, FCI model and HXD-high, HXD-middle, HXD-low dose groups, with 8 rats in each group. T