Blueberry proanthocyanidins on neonatal rat cardiac fibroblasts proliferation.docVIP

 PAGE \* MERGEFORMAT 4 Blueberry proanthocyanidins on neonatal rat cardiac fibroblasts proliferation Author: Shen Nan, Yan-Chun Wang, Ren Kuang, GU Rao Sheng, Fan Hongyan [Keywords:] Proanthocyanidins category; myocardium; fibroblast; nitric oxide; Nitric oxide synthase 0 INTRODUCTION proanthocyanidins (procyanidin, PC) is a blueberry important biological active ingredient, is epicatechin and epigallocatechin table oligomer one or more of the polymer. Some studies [1-3] found that, PC with anti-oxidation , anti-tumor, anti-aging and anti-inflammatory and other effects, but the pharmacological activity of PC blueberry research has been rarely reported. In this study, angiotensin Ⅱ (Ang Ⅱ )-induced cardiac fibroblasts (CFb) proliferation, observed blueberry PC right CFb inducible nitric oxide synthase  nitric oxide (iN0S  NO) the impact of the system to explore the proliferation of PC inhibited CFb initial mechanism. 1 Materials and methods 1.1 Material Wistar rats were 30, 1 ~ 3-day-old (Jilin University, School of Basic Medical Laboratory Animal Center); blueberry proanthocyanidins (Medical Technology Co., Ltd. Nanjing, Solange); Ang Ⅱ and the MTT (U.S. Sigma Company); IMDM, serum (GIBCO Inc.); iNOS primary antibody (Santa cruz companies); trypsin (Po Tektronix biotechnology companies); hydroxyproline kit, iNOS Kit (Nanjing Jiancheng Bio-engineering firm); TGF  β 1 ELISA Kit ( gemmy Bio-Engineering Co., Ltd.); NO Kit (Pik-day Biotechnology Research Institute); SP Kit (Fuzhou taking of new Bio-Technology Co., Ltd.); FITC goat anti-rabbit Ig (Beijing Zhong Shan Golden Bridge Biotechnology Co., Ltd.). 1.2 Methods 1.2.1 Cell Isolation and Culture of Wistar rats were sterile thoracotomy take heart, D  Hank  s liquid cleaning, cut into 1 mm * 1 mm * 1 mm-sized fragments, 20 mL / L repeated trypsin digestion (37 ℃ water bath, 8 min / times), various digestion supernatants were collected, 1000 r / min, centrifuge 5 min, supernatant containing 10 mL