BMP 2 active peptides in vitro induced BMSCs osteogenic differentiation of the experimental study.docVIP

 PAGE \* MERGEFORMAT 25 BMP 2 active peptides in vitro induced BMSCs osteogenic differentiation of the experimental study Abstract Bone morphogenetic protein  2 (BMP  2) has a strong induction of rat bone marrow mesenchymal stem cells (BMSCs) to the direction of orientation osteogenic differentiation capacity. Natural BMP  2 limited number of complex structure limits its wider application. In this experiment were designed and synthesized BMP  2 active peptides, in vitro tests to explore the direction of BMSCs to the osteoblast-directed ability to induce osteogenic differentiation, the evaluation BMP  2 active peptides induced bone effects. [Methods] Experiments were divided into two groups, induction group and the non-induced group. To take a 4-week Wistar rats were isolated and cultured BMSCs, spread to the 3rd generation, the experimental group in favor of osteogenic induction medium (DMEM complete medium BMP  2 active peptides 200 μg / ml). The non-induced group, still using DMEM complete medium. Continue to develop 2 to 4 weeks, using cell seeded culture, alkaline phosphatase activity and calcium content determination, real-time quantitative PCR detection of type Ⅰ collagen (Col  Ⅰ) and osteopontin (OPN) mRNA expression, evaluation of BMP  2 active peptides induce osteogenic potential in vitro. [Results] induced group BMSCs grew well, showing typical osteoblasts similar morphological features and biological characteristics, ALP activity increased, calcium content increased, Col  Ⅰ, and the mRNA was highly expressed OPN. The non-induced group, osteogenic properties was not obvious. [Conclusion] The synthesis of BMP  2 active peptides can effectively contribute to the direction of differentiation of BMSCs to differentiate into bone, is an ideal bone tissue engineering bone inducing cytokines. Has the natural BMP  2 similar to the bone induction activity, and has broad application prospects. Keywords: BMP  2 active peptides of b