Bone marrow mesenchymal stem cells and type Ⅱ collagen-modified PLGA observation of adhesion.docVIP

 PAGE \* MERGEFORMAT 23 Bone marrow mesenchymal stem cells and type Ⅱ collagen-modified PLGA observation of adhesion Abstract Optimization of bone marrow mesenchymal stem cells (bone marrow mesenchymal stem cells, BMSCs) in vitro isolation and culture techniques, improved bio-poly glycolic acid - lactic acid copolymers (poly lactide  co glycolide, PLGA) of the cell adhesion was observed BMSCs and Ⅱ type I collagen-modified PLGA of adhesion. [Methods] density gradient centrifugation BMSCs, flow cytometry analysis (FACS) on the cell surface antigen, cell viability, cell cycle detection, phase-contrast microscope observation of cell morphology. Phase separation method to do PLGA, Ⅱ collagen surface modification, taking the third generation of stem cells and PLGA echo, scanning electron microscopy of cell adhesion and materials. [Results] BMSCs can be isolated in vitro amplification, expression CD29, CD44 and CD106, did not express CD34 and CD45, cell viability of 88.96%, G0  G1 cells accounted for 90.32%. The length of spindle cell morphology, Ⅱ collagen surface modification of PLGA average pore size of 100 μm, with BMSCs have better adhesion. [Conclusion] BMSCs in vitro long-term, stable and training, is an ideal seed cells for tissue engineering. Type Ⅱ collagen-modified PLGA surface with the stem cells have better adhesion can be used for tissue engineering of biological materials. Keywords: bone marrow mesenchymal stem cells in PLGA tissue engineering Abstract: [Objective] To explore a method for isolating and culturing bone marrow mesenchymal stem cell (BMSCs) in vitro, and improve the cellular conglutination ability of biomaterial poly  lactide  co  glycolic acid (PLGA), and observer the adhesion of BMSCs cultured on PLGA modified with Ⅱ collagen. [Method] BMSCs were isolated and cultured in vitro.The cell exterior antigen, cell livingness and cell cycle were analyzed by flow cytometry method, and cellular configuration was obser