Bone marrow-derived mesenchymal cells and rapid-setting calcium phosphate co-culture experimental study stent.docVIP

 PAGE \* MERGEFORMAT 21 Bone marrow-derived mesenchymal cells and rapid-setting calcium phosphate co-culture experimental study stent Abstract [Objective] To observe the human bone marrow mesenchymal stem cells in the fast-setting calcium phosphate bone cement stent in the proliferation and growth of. [Methods] will speed curing of chitosan  calcium phosphate cement scaffold material pre-forming. Separation of whole bone marrow with human bone marrow mesenchymal cells and in vitro on cells cultured and expanded. 72 h for the first time for fluid, cell fusion, 80% of the passage, take the 3rd generation cell used in the experiment. The cells with calcium phosphate cement scaffold training and use, respectively, tetrazolium blue MTT method and scanning electron microscopy detection of cells in the proliferation of materials and growth conditions. [Results] OD values detected by microplate reader tips, after co-culture, the number of stent cells gradually increased. Scanning electron microscopy showed that within the porous scaffold structure, hole saw cell growth, cell surface, secretory granules can be seen. [Conclusion] fast-curing chitosan  calcium phosphate bone cement on human bone marrow mesenchymal stem cells have a better cell compatibility, cell adhesion can be within the material and proliferation, is an ideal bone tissue engineering scaffold material. Keywords: calcium; chitosan; bone marrow mesenchymal cells; tissue engineering; in vitro culture Tissue engineering is a large number of cells in three-dimensional ordering constitute a body while the stent material is ordered three-dimensional arrangement of the cells necessary for the carrier. In bone tissue engineering scaffold material, the calcium phosphate bone cement due to have easy shaping and injection characteristics of the current study to become a hot 〔1〕. After years of toughening, and the curing speed modification, the calcium phosphate bone cement in the clinic