Bupleurum ISSR-PCR reaction system and the establishment and optimization of.docVIP

 PAGE \* MERGEFORMAT 15 Bupleurum ISSR-PCR reaction system and the establishment and optimization of Author: Sui Chun Wei and Chen Shi-Lin Yang Xin-Quan Yang Min [Abstract] Objective To establish a suitable Bupleurum ISSR analysis of PCR reaction system; selection of suitable primers, and determine the best annealing temperature. CTAB method of extraction leaves of DNA, respectively, by single factor experiments to identify suitable ISSR-PCR reaction conditions, using gradient PCR to determine optimal primer annealing temperature. Is suitable for the analysis of the results of PCR Buple