Chfr gene in acute leukemia bone marrow cells in the expression of.docVIP

 PAGE \* MERGEFORMAT 6 Chfr gene in acute leukemia bone marrow cells in the expression of Author: Gong Hui, Liu Li, Jian-Feng Zhou, Ran Dan, Hui-Zhen Xu [Abstract] In order to explore the acute leukemia (AL) in patients with bone marrow cells in early mitosis Chfr checkpoint gene expression and its significance in 46 patients with newly diagnosed untreated AL patients with bone marrow mononuclear cells, using RT-PCR and immunohistochemical methods Detection of Chfr gene mRNA and protein expression and with normal bone marrow mononuclear cells were compared. The results showed that: immunohistochemistry and RT-PCR analysis showed that 28 cases of acute myeloid leukemia (AML) in 15 cases, 18 cases of acute lymphoblastic leukemia (ALL) in 13 cases of bone marrow mononuclear cells Chfr gene mRNA and protein expression decreased significantly compared to normal controls, while cases of Chfr gene expression in abnormal chromosome karyotype abnormalities exist. Conclusion: The acute leukemia bone marrow cells in mitosis checkpoint gene Chfr damage, may play an important role in the pathogenesis of leukemia. [Keywords:] Acute Leukemia Analysis of Expression of Mitosis Checkpoint Gene Chfr in Bone Marrow Cells of Acute Leukemia Patients Abstract This study was purposed to investigate the significance of mitosis checkpoint gene chfr expression in acute leukemia (AL). 2 ml of bone marrow were extracted from each of 46 AL patients and 10 normal donors as control and their mononuclear cells were isolated. Then, their chfr expression was detected by using RT-PCR and immunohistochemistry. Normal control blood samples were also analyzed. The results showed that in 15 out of 28 cases of acute non-lymphocytic leukemia and 13 out of 18 cases of acute lymphocytic leukemia expression of chfr gene mRNA and protein significantly decreased as compared with control. The cytogenetic analysis of patients with a decreased Chfr expression revealed abnormal chromosome. In conclusion,