Child immunization failure S gene of hepatitis B virus a sequence analysis of antigenic determinants.docVIP

 PAGE \* MERGEFORMAT 10 Child immunization failure S gene of hepatitis B virus a sequence analysis of antigenic determinants Of: Yu Qin, LIU Yu-qin WANG Yong-zhong Jiang Jianhua [Abstract] Objective: To investigate the hepatitis B vaccine with high titers of hepatitis B immune globulin PMTCT child immunization failure virus S gene “a” determinant of the variation. Methods: 30 patients with high titer of hepatitis B vaccine hepatitis B immune globulin mother After the failure of infants and blocking immune serum surface antigen (HBsAg-positive children by polymerase chain reaction (PCR amplification of the serum HBV DNA S gene region, and the direct labeling of PCR products were sequenced. Results: 30 patients with HBsAg-positive children 28 patients with serum HBV DNA positive, of which 8 patients had the S gene “a” determinant of variation and mutation rate was 28.6%, the first 126 isoleucine (Ile is threonine (Thr replacement in 2 cases, the first 134 phenylalanine (Phe replaced by isoleucine in 2 cases, the first 145 glycine (Gly is alanine (Ala replacement in 4 cases. Conclusion: The high price of hepatitis B vaccine hepatitis B immune globulin immune failure PMTCT S gene in the presence of children “a” epitope variation. [Keywords:] Hepatitis B virus, hepatitis B vaccine, high titers of hepatitis B immune globulin, mutation, gene [Abstract] Objective: To investigate hepatitis B virus (HBV) S gene “a” determinant mutation in children who failed to be protected by hepatitis B vaccine and hepatitis B immune globulin.Methods: HBV DNA S gene was amplified by polymerase chain reaction and sequenced directly. Results: There were 28 samples for HBV DNA positive in all children with HBsAg positive, eight mutations were detected, the mutation rate was 28.6%; two strain showed Ile to Thr at the 126 codon of surface antigen, two strain showed Phe to Ile at the 134 codon, and four strains showed Gly to Ala at the 145 condon of surface antigen. Conclusion: H