Chinese herbal compound 962 capsule on the aged rat hippocampus nerve growth factor and its receptor expression in.docVIP

 PAGE \* MERGEFORMAT 3 Chinese herbal compound 962 capsule on the aged rat hippocampus nerve growth factor and its receptor expression in Abstract Objective: The pathogenesis of senile dementia, age is the most important risk factors, with age, the incidence rate was significantly increased in aging rats, the model is closer to the pathological changes of Alzheimer’s disease animal model. Our previous results show: Chinese herbal compound 962 capsules (including fleece-flower root, stone Ge Liu Wei Po Chinese medicine, etc.) can significantly improve memory function in aged rats. The observe the effect of 962 capsules of natural aging rats hippocampus CAI-CA4 area of nerve growth factor (NGF) and its receptor expression TrK A to explore the Chinese herbal compound 962 capsules improve learning and memory mechanism. : Female Wistar rats aged 22 months of age, youth is 5 months old. A positive control for piracetam (piracetam) 0.56g/kg, Chinese herbal compound 962 pouches of high-dose 1.8g/kg, 962 capsules low dose 0.9g/kg, once a day gavage, administered orally 2 months to carry out learning and memory test (water maze) and the open field, each group will randomly select 5, perfusion fixed, frozen sections, film thickness 40μm, each specimen were ABC’s method NGF and TrK A immunohistochemical staining. Using French Noesis Vision Inc. VISILOG5.0 image analysis system to histological analysis. Microscope magnification of 10 × 10, in the hippocampus CAl pyramidal cell layer of continuous intake of three horizons, the three horizons, including the entire CAl area, count 3 positive cells within the view of the total number of positive cells in the total area (as Su-units) and the integral value of positive cells in gray (gray units). Hippocampus CA2 area continuous intake of two horizons, hippocampal CA3 area intake of two successive horizons, dentate gyrus of the hippocampus CA4 area intake included a vision, including the CA2, CA3, CA4 area. Data with mean ± stan