Cholesterol stones and apolipoprotein B gene polymorphism.docVIP

 PAGE \* MERGEFORMAT 3 Cholesterol stones and apolipoprotein B gene polymorphism 【Abstract】 Objective To investigate the cholesterol gallstone disease and apolipoprotein (Apo) B gene polymorphism. Methods of apolipoprotein B gene polymorphisms Xba Ⅰ, EcoR Ⅰ polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) technique on 101 patients with cholesterol gallstone disease and 50 controls were studied. Results cholesterol gallstone group X allele frequencies were significantly higher (P lt;0.05), with the X allele of their blood lipids total cholesterol (TC), low-density lipoprotein cholesterol (LDL-c), and ApoB levels were significantly higher than Non-X are (P lt;0.01); cholesterol gallstone group and the control group EcoR Ⅰ allele frequencies and lipid levels did not differ significantly (Pgt; 0.05). Conclusion X allele may be cholesterol gallstone susceptibility genes. Relationship between polymorphisms of the apolipoprotein B gene and cholesterol gallstone disease 【Abstract】 ObjectiveTo investigate the relationship between the apolipoprotein (Apo) B gene polymorphic sites Xbal, EcoRI and cholesterol gallstone disease.Methods Two polymorphic sites Xbal, EcoRI of ApoB gene were selected. Using polymerase chain reaction and restriction fragment length polymorphism technique 101 patients with cholesterol gallstone and 50 controls were investigated.Results The frequency of rare allele X was significantly higher in the patients with cholesterol gallstone than in the controls. Patients with X allele had higher tC, LDL-c and ApoB levels, significantly different from those without X allele. there were no difference between the patient group and the control one in EcoRI allele.Conclusion X allele might