Cholinergic ventral telencephalon precursor cells of embryonic origin.docVIP

 PAGE \* MERGEFORMAT 12 Cholinergic ventral telencephalon precursor cells of embryonic origin [Abstract] Objective To explore cholinergic neurons of embryonic origin, verify whether the expression of radial glial cell acetylcholine transferase. Methods 14 days of gestation (E14 rat embryonic telencephalon coronal paraffin sections were vimentin (radial glia landmark protein, vimentin and acetylcholine transferase (a sign of cholinergic neurons protein ChAT) double immunofluorescence staining, and duplication of the same field by fluorescence staining in the picture to accurately reflect the expression of radial glial cells ChAT position. Results E14 ventral lateral area of rats, some brain tissue was vimentin / ChAT-positive immunohistochemical staining, and the data confirmed that cholinergic neurons appear in the third ventricle E14 days of front and side of the conclusion is the same. Conclusion E14 brain of rats the expression of radial glial cells ChAT. that radial glial cells have neural stem cell properties, which continue to be asymmetric division, resulting in cholinergic phenotype into nerve cells, that bile base can be precursor cells. [Keywords:] cholinergic neurons, radial glial cells, acetylcholine transferase, vimentin Abstract: Objective To explore the embryonic source of cholinergic neurons and to verify whether the radial glial cells can express acetylcholine transferase.Methods Coronary paraffin sections of rat telencephalon on embryonic 14 days (E14) were prepared. Double fluorescent and immunohistochemical staining of vimentin (a protein marker of radial glial cell) and choline acetyltransferase (ChAT, a protein marker of cholinergic neuron) to test whether ChAT was expressed in the population of radial glial cell in vivo. Overlapped pictures in the same field of vision reflected the precise position where the radial glial cells expressed ChAT.Results Vimentin-positive cells coexpressed ChAT in ventral ganglionic eminence in E14