Chronic myeloid leukemia karyotype and the correlation of serum alkaline phosphatase levels.doc

 PAGE \* MERGEFORMAT 8 Chronic myeloid leukemia karyotype and the correlation of serum alkaline phosphatase levels [Keywords:] chronic myeloid leukemia nuclear alkaline phosphatase Chronic myeloid leukemia (chronic myelocytic leukemia, CML is a hematopoietic stem cells derived from malignant disease, clinical into chronic phase, accelerated phase and blast crisis of three processes, in accelerated phase and blast crisis nature of the process of change in disease, the patient Clinical manifestations and treatment are different. We CML patients at different stages of serum alkaline phosphatase (alkaliphosphatase, ALP) and bone marrow cells karyotype were studied to investigate the serum ALP level and clinical stage CML karyotype changes in relationships. are as follows. 1 Subjects and methods 1.1 Case Selection Cases from January 2000 to October 2006 in our hospital out-patient and hospitalization in patients with chronic myeloid leukemia, all patients were diagnosed according to FAB diagnostic criteria, not including co-infection, solid tumor, chronic liver disease and autoimmune diseases in patients with CML . subjects were divided into groups, and CML in chronic phase CML accelerated or blast phase group, and normal healthy control group. CML in chronic phase group of 20 patients, including 12 males and 8 females, aged from 15 to 69 years, median age 42. CML accelerated blast phase 18 cases, including 13 males and 5 females, aged 16 to 70 years, median age 40 years. 1.2 Specimen Collection Patients with fasting blood collected sterile 2 ml, the formation of blood clots after 2 000 r / min the serum was separated after centrifugation 20 min, -80 refrigerator under test. Indicators and methods 1.3 Observation Observation of serum LDH, HBDH (@ hydroxybutyric acid, WBC count, percentage of blast cells in bone marrow. Serum LDH, HBDH directly detected by biochemical methods. WBC, percentage of blast cells in bone marrow blood cells were