Cistanche extract on cell damage model of Parkinsons disease protective effect of.docVIP

 PAGE \* MERGEFORMAT 8 Cistanche extract on cell damage model of Parkinson’s disease protective effect of Author: Wang Hu, Liwen Wei, Ding-Fang Cai, Ru [Keywords:] Cistanche; 1  methyl  4   phenyl-pyridine ion; Parkinson’s disease; survival rate Parkinson’s disease (Parkinson’s disease, PD) is a progressive degenerative diseases of the central nervous system. Once the clinical symptoms, their nigrostriatal dopamine neuron system has been reduced by 60% ~ 80%. While neuroscientists to take into account a variety of agents, including L-dopa treatment, but all current studies have prompted not stop its progress. Growth inhibition and DNA damage inducible gene 153 (growth arrest  and DNA damage  induced gene 153, GADD153) is the regulation of apoptosis is an important protein in the cell such as endoplasmic reticulum stress-stress state of a large number of expressed and transferred in the nucleus [1]. This study observed the extract of Cistanche methyl-1    4  phenyl-pyridine ion (1  methyl  4  phenylpyridinium ion, MPP)-mediated SH  SY5Y dopaminergic cell line injury and the expression of GADD153 effects and discussed its neuroprotective effect of Parkinson’s disease. 1 Materials and methods 1.1 Materials Cistanche extract, Shanghai Oriental technology company’s products, PBS diluted to 10 mg / ml, 22 μ m membrane filter sterilization; MPP, Sigma’s products, sterile distilled water diluted to 4 mol / L; fetal bovine serum, Dahl Burke modified Eagle medium (Dulbecco’s modified eagle’s medium, DMEM), trypsin, were Gibco products; GADD153 mouse anti-human monoclonal antibody, Santa Cruz company’s products; mouse anti-human β  actin monoclonal antibody , Sigma company’s products; methyl thiazolyl tetrazolium (methyl thiazolyl tetrazolium, MTT), the Netherlands Duchefa company’s products; reverse transcription kit (reverse transcriptase  polymerase chain reaction, RT  PCR), MBI company’s products; SH  SY5Y cells, State Key