Citation Yang Capsules Determination of icariin.docVIP

 PAGE \* MERGEFORMAT 6 Citation Yang Capsules Determination of icariin [Keywords:] icariin; quoted Yang Capsule; high performance liquid chromatography Abstract: Objective To establish cited Yang Capsules icariin high performance liquid chromatography (HPLC) assay method. Methods HPLC method. Column Lichrospher C18 (4.6mm * 250mm, 5μ m), C18 guard column; mobile phase of acetonitrile 20g / L acetic acid aqueous solution (30:70); detection wavelength 270nm; flow rate 1.0mL/min; column temperature was room temperature; injection volume of 10μ L. Sample plus 50% (volume fraction) ethanol ultrasonic extraction, extract measured after over 0.45μ m membrane. Results icariin linear range of 10.0-60.0mg / L, the regression equation is C = 2.042 * 10-5A 0.554, r = 0.9993, average recovery was 99.8%, RSD was 0.65%. Conclusion This method is used to cited Yang Capsules Determination of icariin, when simple and quick and accurate. Keywords:: icariin; quoted Yang Capsule; high performance liquid chromatography Determination of icariin in Yinyangsu capsules by HPLC ABSTRACT: Objective To establish an HPLC method for testing the content of icariin in Yinyangsu capsules. Methods An Lichrospher C18 (4.6mm * 250mm, 5μ m) was used as the column, and column C18 as the protecting column. The mobile phase was a mixture of acetonitrile-20g / L acetum (30:70) in a flow rate of 1.0mL/min. The detected wavelength was 270nm. Column temperature was room temperature and the injection quantity was 10μ L. The sample was prepared when we added 50% ( V / V) alcohol and then made ultrasonic extraction. Measurement was made after the prepared sample was filtrated on 0.45μ m filter paper. Results Icariin in the sample was well separated. The linear range was 10.0-60.0mg / L. The linear equation was C = 2.042 * 10-5A 0.554, while the correlation coefficient was 0.9993; the average recovery was 99.8% and RSD was 0.65% (n = 6). Conclusion This method can be used in content measurement of Yinyan