Chua Fuzheng Decoction on SGC-7901 gastric cancer cell adhesion molecule expression.docVIP

 PAGE \* MERGEFORMAT 12 Chua Fuzheng Decoction on SGC-7901 gastric cancer cell adhesion molecule expression Of: Lang Wei, Wang Haibo, WU Jian-bo, Zhang Sheng Hui, Cai Shen early Yin Lihui [Abstract] Objective: To investigate the righting Chua Decoction on the growth of cancer cells outside the body surface of SGC-7901 and the role of adhesion molecules in the measurement and effect relationship. Methods: Flow cytometry detection of cell surface E-Cadherin, CD44, CD44-v6 and CD54 expression. Results: The recipient of the role of righting Soup After 48 h, SGC-7901 cell membrane increased expression of E-Cadherin, CD44, CD44-V6, and CD54 expression was decreased. As the dose increases, E-Cadherin positive cell expression gradually increased; CD44, CD44-v6, CD54 expression positive rate decreased, the inhibition rate increased gradually. Conclusion: Chua Fuzheng Soup in inhibition of human gastric cancer SGC-7901 cell proliferation, based on the cancer can increase SGC-7901 cell surface expression of E-Cadherin, reduce gastric cancer SGC-7901 cell surface CD44, CD44-V6 and CD54 expression, and there is a link between measurement and effects, that can stop Chua righting Soup SGC-7901 gastric cancer cells adjacent normal tissue invasion and distant metastasis, and thus play a role in cancer. [Keywords:] Traditional Chinese Medicines; gastric cancer cells; E-cadherin; CD44; CD54 Abstract: Objective: To investigate the effects as well as dose and effects relationship of Prescription of Cai-shi-fu-zheng-xiao-zheng-Tang on the proliferation and cell adhesion molecule changes of human gastric carcinoma cell line SGC-7901 in vitro . Methods: Flow cytometry was used to analyze the expression of cell adhesion molecules, including E-Cadherin, CD44, CD44-V6 and CD54. Results: Cai-shi-fu-zheng-xiao-zheng-Tang inhibited the proliferation of SGC- 7901 cells in a dose-and time-dependent manner. with the expression of E-cadherin increased concomitant with CD44, CD44-V6