Chromosome mosaicism in preimplantation embryos of a preliminary analysis of.docVIP

 PAGE \* MERGEFORMAT 26 Chromosome mosaicism in preimplantation embryos of a preliminary analysis of 【Abstract】 Objective fluorescence in situ hybridization (FISH) technology to human chromosome mosaicism of early embryos to conduct a preliminary analysis and to explore the embryonic chromosome mosaicism in conventional in vitro fertilization - embryo transfer (IVF-ET), as well as the success rate of implantation the impact of pre-operative diagnosis. Methods IVF-ET treatment cycles in embryos of poor quality, not suitable for embryo transfer and cryopreservation of normal fertilized embryos as well as in the diagnosis of preimplantation embryos diagnosed as male sex embryos can not be transplanted in 38 embryos FISH. Results A total of 293 embryos of 38 nuclear, hybrid rate was 96.2%. One normal embryo 12, accounting for 31.6%, diploid embryos (normal embryonic diploid chimeric embryos) 28, accounting for 73.7%, the chimeric embryos (diploid chimeric embryos abnormal chimeric embryo) 19, accounting for 50.0%. In the ≤ 4-cell stage embryos, the chimeric embryos 18.2%, but in 5 ~ 8-cell stage, and ≥ 9-cell stage embryos, the chimeric embryos to increase the proportion of 68.4%, respectively, and 50.0% respectively. Conclusion With the increase in the number of cleavage, the proportion of chimeric embryos also increased, but the chimeric embryos, the proportion of chimeric cells was correspondingly lower. Embryo chromosome mosaicism are likely to affect the success rate of conventional IVF-ET the important factor. Application of FISH technology and accurate single-cell preimplantation gender diagnosis, but with a single pre-implantation genetic diagnosis, it is best to biopsy two cells at the same time in order to increase accuracy. Preliminary Analysis of Chromosome Mosaicism in Preimplantation Embryos 【Abstract】 Objective Using fluorescence in situ hybridization to analyze chromosome mosaicism in human preimplantation embryos