Chromosomal Location of Genes.docVIP

 PAGE \* MERGEFORMAT 17 Chromosomal Location of Genes Keywords:: gene mapping by fluorescence in situ hybridization radiation hybrids In the Human Genome Project (HGP) has most of two, one in 2005, before the completion of human genome DNA of about 3 * 109 nucleotide sequence determination, and chromosome location of genes to complete the work; second is to carry out gene Function. Gene mapping and gene sequence of the two complement each other and the positioning of both chromosome genes contribute to the determination of gene sequences is also beneficial to the gene structure and function studies will help further tips of biological genetic information. Gene sequencing technology, in particular the establishment of large-scale sequencing technology, which greatly improved the speed of gene sequencing. To January 25, 1999 date, the world has to detect all human gene sequences to 7.3%, while some organisms, such as yeast, E. coli and other sequences have been determined complete, so that the work of Chromosomal Location of cDNA is particularly urgent. However, due to the current large-scale genome sequencing is built on the basis of gene mapping and genome chromosomal location (gene mapping) work to keep up gene sequencing technology development, and it became a limited gene sequencing of the main factors. Chromosomal Location of Genes are various ways that it can be divided into genetic mapping and physical mapping and physical mapping of the most important is the fluorescence in situ hybridization (FISH) and radiation hybrids (RH) two kinds. This paper present the main methods of gene mapping for an introduction. A fluorescence in situ hybridization (FISH) In situ hybridization (ISH) of the original long been used for karyotype analysis and nucleic acid distribution, and later, as technology develops, it is used in genetic chromosome mapping studies, in particular the development of non-isotope labeling technique, allowing the application of ISH has a wid