Column derivatization high performance liquid chromatography mass spectrometry Nick nicotinamide content in rice.docVIP

 PAGE \* MERGEFORMAT 13 Column derivatization high performance liquid chromatography mass spectrometry Nick nicotinamide content in rice Of: Science Cao Ming Zhao Mu Renxiang [Abstract] established a pre-column derivatization high performance liquid chromatography mass spectrometry (HPLC MS) determination of content of rice in the way Nick nicotinamide. Nicotinamide Nick samples extracted by water, and 9 fluorene methyl chloroformate (FMOC Cl derivative by liquid chromatography mass spectrometer measurement. systematically studied and derived media concentration derived conditions on the derivatives efficiency. acidity and by optimizing the mobile phase gradient elution conditions, improve the sensitivity of the method. Nick NICOTINAMIDE 0.1 ~ 5.0 mg / L range was a good linear relationship (r = 0.9983, on rice roots, stems, leaves and rice standard addition experiments show that the method recoveries were 72.0% ~ 89.2%, the relative standard deviation of 2.3% -9.6%, and the detection limit was 0.05 mg / kg. The method is simple, accurate and reliable, can meet the physical level in rice Nick qualitative and quantitative analysis of nicotinamide. [Keywords:] Nick nicotinamide, rice, pre-column derivatization, high performance liquid chromatography, mass spectrometry Determination of Nicotinamide in Rice by Pre column Derivatization and High Performance Liquid Chromatography Mass Spectrometry CAO Zhao Yun, MOU Ren Xiang, CHEN Ming Xue (Rice Product Quality Inspection and Supervision Center, Ministry of Agriculture, China National Rice Research Institute, Hangzhou 310006) Abstract A method was developed for the determination of nicotinamide (NA) in rice by pre column derivatization and high performance liquid chromatography / electrospray ionization mass spectrometry (HPLC / ESI MS). After extraction with water, NA was derivatized by 9 fluorenylmethoxycarbonyl (FMOC Cl ). In order to enhance the sensitivity, the derivatization was optimize