Combined detection of anti-nuclear antibody anti-ENA spectrum anti-dsDNA antibodies on the clinical value of SLE.docVIP

 PAGE \* MERGEFORMAT 11 Combined detection of anti-nuclear antibody anti-ENA spectrum anti-dsDNA antibodies on the clinical value of SLE Of: Bin Luo, Tseng Yung-Lung, Huang Wei, Wei Caicheng [Abstract] Objective To investigate the combined detection of anti-nuclear antibodies (ANA, anti-extractable antigen (anti-ENA and anti-double-stranded DNA antibodies (anti-ds DNA in systemic lupus erythematosus (SLE) clinical value. ANA using indirect immunofluorescence method detection, anti-ds DNA antibodies with colloidal gold dot penetration assay, anti-ENA spectrum by Western blot. Results SLE group ANA positive rate was 94.44%, anti-ds DNA antibody positive rate was 63.33%, anti-Sm antibody positive rate was 50.00% the control group, only ANA positive rate was 6.67%, other antibodies were negative. Conclusion ANA, anti ds DNA antibodies and anti-Sm antibodies can be used as a symbol of SLE antibody detection, joint detection can avoid the situation of single missed detection occurs to improve the detection rate of SLE, the diagnosis of the disease type, treatment, and prognosis are important to track the value. [Keywords:] systemic lupus erythematosus antinuclear antibody anti-ds DNA antibodies anti-ENA Systemic lupus erythematosus (SLE is a common multi-system autoimmune connective tissue disease damage, and its remarkable performance of the clinical features of a high degree of activation of B lymphocytes produce a variety of organizations, including against its own nucleus and various nuclear components, cell membrane, cytoplasm autoantibodies against a variety of tissue components, detection of these autoantibodies, especially combined detection of anti-nuclear antibody (ANA), anti-salt extractable nuclear antigen antibodies (anti-ENA), anti-double-stranded DNA antibodies ( Anti-ds DNA) diagnosis of SLE is very important. We retrospectively analyzed 90 patients with SLE of ANA, anti ds DNA antibodies, anti-ENA testing results, and with 30 healthy per