Common features of neural tracer and the tracer.docVIP

 PAGE \* MERGEFORMAT 17 Common features of neural tracer and the tracer OF: He Li Li Zhao Lei Make Tao Zhu Division Army Strong [Abstract] rich neural tracing technique greatly promote the development of neural anatomy, for a variety of neurobiology provides a good foundation in this, we outlined the common features of neural tracer and the tracer, focuses on a variety of fluorescent dyes and IB4 lectin tracing features. [Keywords:] neural tracer; horseradish peroxidase; fluorescent dye; plant lectin IB4; virus Since the 20th century, the first time the early 70s Kristenson horseradish peroxidase (HRP) applied to track nerve fiber link has been made in the research of the aspects of the unprecedented rapid development. Since then, many widely used, the sensitivity of strong and can choose sexual conduct anterograde, retrograde, or both cis, retrograde tracing substances were applied to the nerve fiber connection, the development of neural anatomy played a positive role. now commonly used neural tracer and Tracer characteristics are summarized as follows: A horseradish peroxidase 1.1 horseradish peroxidase (Horseradish peroxidase, HRP) HRP is a heme-based plants containing glycoprotein. HRP method is the development of the 20th century and was widely used 70 years of a neural tracing method, but the development needs of the HRP many complex immunohistochemical technique, and the HRP involved in cell metabolism, can not long persist in cells, easily spread to nearby tissue staining caused by misuse of neurons, and its reaction products more unstable, and easily lost, there is also “re-uptake” phenomenon [1], making the HRP retrograde tracing in the application of neural greatly reduced. 1.2 The combination of cholera toxin B subunit horseradish peroxidase (CB HRP) RNRanson [2] on the traditional horseradish peroxidase staining method was improved by a combination of cholera toxin B subunit horseradish peroxidase (CB HRP) as tracer, clearly sh