Cordyceps mycelia drug serum on interleukin-1β injury in the protection of pancreatic islet cells in rats.docVIP

 PAGE \* MERGEFORMAT 19 Cordyceps mycelia drug serum on interleukin-1β injury in the protection of pancreatic islet cells in rats Author: Zheng Qian Liu Yong Liu Lan Haitao Cao Di Jing Hua E [Abstract] Objective interleukin-1β (Interleukin-1β , IL-1β ) injury in isolated islet cells, study of Cordyceps sinensis (Cordyceps sinensis, CS)-containing serum on islet cells and its mechanism of protective effect of antioxidant enzymes and nitric oxide (Nitric oxide, NO) relationship. Method of in vitro cultured neonatal rat islet cells with IL-1β damage islet cells with serum containing different concentrations of CS were incubated together, the application MTT cell viability assay, radioimmunoassay and high-sugar-based insulin secretion, colorimetric detection of cell supernatant of nitric oxide (NO) levels, inducible nitric oxide synthase (Nitric oxide synthase, iNOS), superoxide dismutase (superoxide dismutase, SOD) and glutathione peroxidase (glutathione perioxidase, GSH-Px) activity. The results of CS-containing serum significantly increased IL-1β damage islet cell activity, and islet cell-based and high-glucose-stimulated insulin secretion was significantly increased. After the effects of IL-1β in cell culture supernatant increased NOS activity, NO content increased, while SOD, GSH-Px activity levels decreased with different concentrations of CS, after co-incubated with serum, cell culture supernatant NO content decreased, iNOS activity decreased, SOD, GSH-Px levels increased significantly in a dose-dependent, statistically significant (P amp;lt;0.05, P amp;lt;0.01). Conclusion CS-containing serum on IL-1β secretion function of islet cell injury and cell activity has a protective effect, the mechanism and reduce the activity of iNOS, thereby reducing NO generation and increased SOD, GSH-PX activity related to the level. [Keywords:] Cordyceps sinensis islet cell damage in serum pharmacological cytoprotection Abstract: ObjectiveTo investigate the effects