内分泌及化学治疗药药理实验的联系与区别.docVIP

内分泌和化学治疗药药理实验的联系与区别 The links and differences between the pharmacological experiments of Endocrine system and chemotherapy drugs 摘要 药理学是研究药物与机体相互作用及作用规律的一门学科。就今天药物的发展我们可以按药理学可大体分为：外周神经系统药、中枢神经系统药、自体活性物质类药、内脏系统要、内分泌系统药、化学治疗药、影响免疫功能药等。药理学的研究与生物系统的背景知识密切相关。随着细胞生物学与生物化学研究的发展，药理学领域的研究内容也在不断改变、充实与完善。通过对药物受体在分子水平上的分析，根据特定细胞信号传导或代谢途径进行药物设计已经成为可能。这种药物设计通常针对细胞表面的能调控或介导细胞信号传导途径从而控制细胞功能的受体。 本实验设计主要讨论内分泌系统药和化学治疗药在药理学实验方法、实验周期以及实验所用材料的不同进行比较和分析。 关键字：药理学实验，内分泌系统药，化学治疗药 abstract Pharmacology is the study of drug interactions with the body and the role of the law of a discipline . To now the development of drug , it can be divided by pharmacological into : Peripheral nervous system drugs , central nervous system drugs , active substances from the body of medicines , visceral system to the endocrine system drugs , chemotherapy drugs , drugs affecting the immune function and so on . Pharmacological research is closely related to background knowledge of biological systems . With the study of cell biology and biochemistry of development , Pharmacological research in the field content is also changing , to enrich and improve . By the molecular level of analysis of receptors of drugs , it has become possible according to a specific cell signaling or metabolic pathways for drug design has become possible . The drug design control cell function in receptor is usually aimed at the cell surface to control or mediated signal transduction pathway . The experimental design focuses on the endocrine system drugs and chemotherapy drugs in pharmacology experimental method, test cycles and test the different materials used for comparison and analysis . Keywords : Experimental Pharmacology , Endocrine system drugs , Chemotherapy drugs 第一章 实验方法联系 1、药理学研究新技术 药理学的目的是找到可以治疗适应症的药物的有效成分。完成初步对药物成分的检测，包括色谱技术、电生理学技术、分子生物学技术、电镜技术、放射自显影技术、放射免疫分析技术等。 无论是内分泌系统药还是化学治疗药在检测其中有药效的化学成分时都要用到气相色谱―质谱联用（GC―MS）、高效液相色谱―质谱联用（LC―MS）、毛细管电泳―质谱联用（CE―MS）等新技术这些技术可以在进行动物实验后很快的检测出有效成分的化学结构和含量 （1）气相色谱―质谱联用气相色谱仪器将复杂混合物试样