Cultured human periodontal ligament cells in vitro method of.docVIP

 PAGE \* MERGEFORMAT 15 Cultured human periodontal ligament cells in vitro method of Author: Zhang Li, Wang, Zhang Mei, Hou Yu, Wu Chenglong, Wen-Hsiu Wang [Abstract] Objective: To study how to improve the in vitro cultured human periodontal ligament cells (hPDL) success rate, for simple, quick access to a large number of high survival rate of hPDL membrane cells to establish in vitro model provides a certain degree of experimental methods. Methods: Using 3 different methods (tissue block method, the enzyme digestion method, the whole tooth digestion method) in periodontal ligament cells in primary culture, using morphology, immunocytochemistry staining methods such as identification of cell origin; through the growth curve Determination of in vitro cell growth characteristics. Results: The three kinds of methods to obtain the cell morphology and biological behavior of roughly the same; Bosi antibody staining, keratin antibodies were negative. Explant cultured cells homologous good, but the beginning of culture a long time; full teeth digestion of the primary culture of success rate is higher than the other two kinds of methods. Conclusion: The periodontal ligament cells through the improvement of culture methods, can significantly improve the hPDL the success rate of primary culture. [Keywords:] periodontal membrane; cell culture; immunohistochemistry [Abstract] Objective: To establish a simple, fast, and efficient technique for human periodontal ligament (hPDL) cell in vitro cultivation with higher success rate. Methord: Primary hPDL cells were cultured using three different methods (tissue explant, explant with enzymatic digestion , and tooth enzymatic digestion). Morphological analysis and immunocytochemical staining were used to characterize the cell lineage, and growth curve assay was adopted to evaluate the biological features of hPDL cells. Results: Morphological and biological characteristics of cells cultured with the 3 kinds of method were simila