Curcumin liposomes and in vitro studies of lipid peroxidation.docVIP

 PAGE \* MERGEFORMAT 13 Curcumin liposomes and in vitro studies of lipid peroxidation [Abstract] Objective To prepare curcumin liposomes and investigate the in vitro lipid peroxidation in rat tissues. Methods prepared by film dispersion extrusion curcumin liposomes, micro-column centrifugation method for the determination of curcumin lipid body encapsulation efficiency (encapsulation efficiency, EE), and the encapsulation rate as the index prescription through the orthogonal filter. using thiobarbituric acid (TBA) assay in rats lipid peroxidation products malondialdehyde aldehyde (MDA) of the formation to determine the effect of curcumin on liposomal lipid peroxidation in rat tissues. The results prepared by the best prescription liposome entrapment efficiency of curcumin (89.32 + -1.58)%. In In vitro, curcumin liposomes on rat heart, liver, kidney, brain lipid peroxidation inhibition rates were 70.02%, 59.48%, 59.10%, 85.21%. and in the experimental range with curcumin liposomes the increase of dosage, the inhibition rate increase. Conclusion extrusion film dispersion of curcumin liposomes prepared simple, curcumin liposomes in vitro lipid peroxidation in rats significantly inhibited. [Keywords:] curcumin; liposomes; entrapment efficiency; orthogonal experimental design; lipid peroxidation [Abstract] Objective To prepare curcumin liposomes and study its impact on the lipid peroxidation in rat tissues in vitro.Methods Curcumin liposomes were prepared by film dispersion and extrusion technique.Then encapsulation efficiency (EE) of curcumin liposomes was determined by minicolumn centrifugation method. Orthogonal experimental design was used to optimize the formulation.To evaluate the impact of curcumin liposomes on the lipid peroxidation in rat tissues, thiobarbituric acid (TBA) method was adopted to detect the amounts of malondialdehyde (MDA), a product of lipid peroxidation reaction. Results The average EE of curcumin liposomes prepared according to th