Demineralized bone - PLA re-development of artificial bone and animal safety evaluation.docVIP

 PAGE \* MERGEFORMAT 15 Demineralized bone / PLA re-development of artificial bone and animal safety evaluation Study: King Liu, Maze Hui, Deng Bing, Pan Xianming, Liu Dong-Fa, Deng Shaolin, Huang Gang, Rong Weiping [Abstract] Objective To develop the artificial bone material with excellent performance, evaluate the relevant physical properties and animal safety. Methods demineralized bone, polylactic acid, sodium chloride according to the proportion of composite, prepared by re-molding enhanced bone (BPCB materials, Detection of porosity, pore size, mechanical strength and other properties of biological parameters, and safety evaluation of animal materials. Results BPCB materials developed porosity 55.20%, pore size 227.33m, compression strength of 5.52MPa, bending strength 19.61MPa , acute toxicity experiments showed that extracts of animals after the injection, without any unhealthy behavior, thermal results show that in each of the original material in the first test of New Zealand white rabbits, body temperature were 0.2 below, and the total degree of body temperature 1.0 in the following, in line with the evaluation criteria pyrogen test, hemolysis test showed hemolysis rate was 0.335 percent, less than 5%, in line with medical materials hemolysis experiments. Conclusion BPCB materials, porosity, pore size and biomechanical strength performance are in line with the requirements of bone substitute materials, and have basic animal safety. [Keywords:] bone substitute materials, artificial bone, demineralized bone, polylactic acid, biocompatibility Development and looking for the ideal bone substitute materials, medical and biological materials has been an important topic in science. In the past, we have designed using the “molded Enhancement Act,” the pearl layer and the combination of two materials, polylactic acid, prepared by Pearl layer / polylactic acid recombinant bone [1,2,3]. Over the years, decalcified bone (demineralized bone) and pol