Detection of human papillomavirus Progress.docVIP

 PAGE \* MERGEFORMAT 11 Detection of human papillomavirus Progress [Keywords:] cervix neoplasms; papilloma virus, human milk Cervical cancer is one of the common gynecological malignancies, the incidence rate of malignant tumors in women ranked second only to breast cancer [1]. In recent years, the upward trend in the incidence of cervical cancer, and getting younger and younger [2]. Man first found that human papilloma virus (HPV), molluscum can be caused by a virus, was later designated as the International Center for Research on Cancer and related cervical lesions caused by key factors [3], in 99.7% of cervical cancer can be seized the HPV [4]. HPV is the most human oncogenic virus diversity in a class, has been identified more than a type, the World Health Organization published in 2005 in 15 HPV types are carcinogenic [5], the main lead to invasive cervical cancer [3]: HPV16, HPV18, HPV31, HPV33, HPV35, HPV39, HPV45, HPV51, HPV52, HPV56, HPV58, HPV59, HPV68, HPV73 and HPV82, such as high-risk HPV.Zuan [6] studies suggest that a high degree of disease combined with cervical HPV16, HPV18, HPV33 or HPV45 infection when the disease is greatly increased the likelihood of progression to cervical cancer. in human cervical cancer samples HPV-DNA detection rate of up to 99%, of which HPV -16, HPV-18 positive rates were 60%, 14% [7], therefore the accurate detection of infection can increase the sensitivity of screening for cervical precancerous lesions. now at home and abroad on the HPV detection method Research Development as follows. 1 HPV detection methods 1.1 The traditional method for cytological examination 1.1.1 cytology cytology for detecting HPV infection in a simple and economical method, commonly used in the screening examination HPV. It caused by HPV infection of the host cell morphological changes and infer the occurrence of HPV infection, method of clinical application of more traditional Pap smear, but because of its limitations have b