Detection of FHIT gene in exfoliated cells in sputum in the evaluation of early diagnosis of lung cancer.docVIP

 PAGE \* MERGEFORMAT 14 Detection of FHIT gene in exfoliated cells in sputum in the evaluation of early diagnosis of lung cancer Of: Zhuangpeng Hui, Jiang Xiaogang, Pan Chengen, yet East [Abstract] Objective To detect the FHIT gene in exfoliated cells in sputum microsatellite instability (microsatellite instability, MSI) and loss of heterozygosity (loss of heterozygosity, LOH), Evaluation of FHIT gene in the early diagnosis of lung cancer detection sensitivity of effective method for lung cancer screening. method based on liquid-based cytology, sputum collection and separation of exfoliated cells of high-risk populations, extracting DNA, detection of FHIT gene MSI and LOH. The results appear in patients with lung MSI or LOH positive rate of abnormal 41.6% ~ 49.5%, with the highest D3S1300, reaching 49.5%, 3 sites in at least one abnormal microsatellite loci was 72.3% (n = 73), at least 2 abnormal microsatellite loci is 45.5 % (n = 46). statistical analysis showed that, FHIT genes in the MSI or LOH in lung cancer and non cancer patients were significantly (P lt;0.05). Conclusions based on liquid-based cytology, testing sputum off cell FHIT gene LOH and MSI can be used as new ways of early diagnosis of lung cancer. different microsatellite loci specific forms of abnormal performance is different, a number of points combined detection can improve the diagnostic sensitivity and specificity. [Keywords:] sputum cytology microsatellite instability diagnosis of lung cancer loss of heterozygosity ABSTRACT: Objective By detecting microsatellite instability (MSI) and loss of heterozygosity (LOH) in FHIT gene in sputum specimen, we intended to evaluate the sensitivity of FHIT gene detection to early diagnosis of lung cancer and explore effective method to screen lung cancer. Methods Based on liquid based cytology, we collected and isolated sputum specimens in high risk group, abstracted DNA, and detected MSI and LOH in FHIT gene. Results The positive rate of MS