Detection of telomerase activity in Progress.docVIP

 PAGE \* MERGEFORMAT 24 Detection of telomerase activity in Progress Abstract: In recent years, studies have shown that the incidence of telomerase activity and tumor development is closely related speculated that it might be a wide range of tumor markers. In this paper, the principle of detection of telomerase activity, telomerase extraction methods, TRAP amplification techniques, the results of four kinds of analytical methods (isotopic method, staining method, fluorescence method and ELISA method), as well as how to conduct quality control and semi-quantitative analysis carried out Overview. Telomerase (Telomerase) is composed of RNA and protein as a ribosomal nuclear protease. Human telomerase RNA component (hTR) was cloned in 1995, which contains the template for telomere repeat sequences (5’-CUAACCCUAAC-3 ‘); protein components with the RNA-dependent DNA polymerase activity, the structure is not clear. Telomerase RNA template can be its own zone as a template to copy synthesis of telomere sequences. In the embryonic cell proliferation such as active cells that have telomerase activity in normal mature somatic cells telomerase inactivation, but also found that the vast majority of telomerase-positive tumor cells were tested, and in the adjacent tissues and The positive rate of normal tissues is very low, speculated that telomerase may be a wide range of tumor markers [1]. Therefore, in recent years, telomerase activity in a variety of tumors of the increasing depth, while promoting improvements in detection methods and improved. This article summarized below its detection methods. A basic principle of Telomerase in vitro RNA templates may be in their own area as a template, in the appropriate oligonucleotides end of the chain with 6 bp repeat sequence, using polyacrylamide (PAGE) gel electrophoresis can be displayed 6 bp differences ladder. Kim was established in 1994, based on the PCR-based telomeric repeat amplifi