Detection of Pseudomonas aeruginosa metal β Comparison lactamase.docVIP

 PAGE \* MERGEFORMAT 8 Detection of Pseudomonas aeruginosa metal β Comparison lactamase Study: Qian Yuan Yao Shu-fen Zheng Yuqiong Wang Peifen [Abstract] Objective: To investigate the detection of metallo-β lactamase and reliable method. Methods: The clinical isolates of imipenem-resistant Pseudomonas aeruginosa producing IMP 1 8 strains and metallo-β-lactamase reference strain one, respectively disk synergy method and the double disc synergy of metal enzyme phenotype assay to compare the results. Results and Conclusion: disk synergy method prone to false positive results, and disk synergy method is relatively reliable method of detection of metal enzymes. [Keywords:] Pseudomonas aeruginosa; metal β-lactamase; resistance Hydrocarbon mildew resistant vinyl antibiotics is the treatment of multiple infections caused by Pseudomonas aeruginosa the most effective antibiotic, but the metal β-lactamase (MBL) production of such a serious threat to the use of antibiotics, therefore, MBL detection has important clinical significance, and the detection of MBL Society for Clinical Laboratory Standards (CLSI) there is no recommended method. We collected the First Affiliated Hospital of Shantou University, clinical isolates of imipenem (IMP) resistant Pseudomonas aeruginosa 8 strains of Aeromonas and MBL producing IMP 1 standard strains of one type, were used disk synergy method and double disk synergy test detected MBL phenotype of P. aeruginosa MBL detection method. 1 Materials and methods Strains were collected 1.1 First Affiliated Hospital of Shantou University Medical College from 2005 to 2006 on the separation of IMP 8 strains of Pseudomonas aeruginosa resistant, double-disk synergy test (2-mercaptopropionic acid as an inhibitor) and PCR, confirmed not produce MBL. producing IMP 1 type MBL as a positive control of Pseudomonas aeruginosa strains, kindly provided by the Professor Yu Yunsong. 1.2 Preparation of bacteria in LB broth overnight culture pl