Determination of cold on the heat particles 5-0-- A 基维斯阿米 glycosides content.docVIP

 PAGE \* MERGEFORMAT 8 Determination of cold on the heat particles 5-0-- A 基维斯阿米 glycosides content [Abstract] Objective To determine the cold heat particles 5-0-- A基维斯阿米glycosides content. Methods HPLC, Hypersil C18 column (4.6 mm × 250 mm, 5 μm); methanol - water (44:56) as mobile phase, flow rate 1.0 mL / min, detection wavelength: 292 nm. Results 5-0-- A 基维斯阿米 glycosides in the range of 0.092 2 ~ 0.922 μg showed a good linear relationship (r = 0.999 9), the average recovery was 97.9%, RSD = 0.71% (n = 6). Conclusion The method is simple, accurate, reproducible and can be used for quality control of cold heat particles. [Keywords:] cold heat particles; 5-0-- a基维斯阿米glycosides, HPLC, determination Abstract: Objective To establish a method for determining the content of 4’-O-β-D-glucosyl-5-O-methylvisamminol in Ganmao Qingre Granule by HPLC. Method The seperation was performed in Hypersil C18 colunm (4.6 mm × 250 mm , 5 μm) with the mobile phase of methanol-water (44:56). The flow rate was 1.0 mL / min and the wavelength was set at 292 nm. Results The calibration curves was linear in the range of 4.608 ~ 46.08 μg / mL for 4’-O-β-D-glucosyl-5-O-methylvisamminol (r = 0.999 9). The average recovery was 97.9%, RSD = 0.71% (n = 6). Conclusion The method is simple, reproducible and can be used for the quality control of Ganmao Qingre Granule. Keywords:: Ganmao Qingre Granule, 4’-O-β-D-glucosyl-5-O-methylvisamminol, HPLC, content determination Received a cold heat particles contained in the 2005 edition of lt;lt;Chinese Pharmacopoeiagt; “(a), by the Nepeta ear, wind, Bupleurum, Pueraria composed of 11 herbs flavor, with a wind-dispersing cold, heat effect of relieving the clinical use seen in the cold cold headache, fever, aversion to cold, body pain, runny nose, cough and throat infections. the original standards of puerarin in puerarin content determination method [1]. Cold heat particles to enhance quality control standards to ensure the stability o