Dgalactose induced senile rats changes in non-enzymatic glycation and its role in alcohol extract of Cuscuta Research.docVIP

 PAGE \* MERGEFORMAT 11 Dgalactose induced senile rats changes in non-enzymatic glycation and its role in alcohol extract of Cuscuta Research Of: Wei Xiaodong Liu Yuping Li Jing Sun Jie parsley [Abstract] Objective D galactose induced senile rats the role of non-enzymatic glycation and its alcohol extract dodder, and to explore the mechanisms of aging and the role of anti-aging mechanism of Cuscuta. Methods D galactose aging rats produced , 15,30,45 d observed when glycated hemoglobin (GHb), malondialdehyde (MDA), superoxide dismutase (SOD) activity changes. semiquantitative RT PCR method glycation end of rat thoracic aorta the end product receptor (RAGE) mRNA expression, and to observe its effect of alcohol extract dodder. Results D galactose GHb, MDA, and RAGE mRNA levels (P lt;0.01), SOD activity decreased (P lt;0.01), and showed progressive changes in time. dodder alcohol extract of the rat GHb, MDA decreased (P lt;0.01), SOD activity increased (P lt;0.01), RAGE mRNA expression levels decreased (P lt;0.01). Conclusion of non-D galactose enzymatic glycosylation occurs, dodder alcohol extract inhibited D galactose induced senile rats non-enzymatic glycosylation reaction, in the administration of 30d that have significant effect. [Keywords:] Dodder; non-enzymatic glycation; aging; D galactose; rat Aging non-enzymatic glycation theory of protein glycosylation caused by cross-linking of injury is the main reason of aging (1,2), the reaction is carried out slowly in the normal body, biological macromolecules can be without the participation of enzymes and amino acids reaction of amino or nucleic acid to form the final end products of advanced glycation (AGE) (3). studies have shown that with aging occur in many tissues the increase in AGE, indicating that AGE in aging and age-related diseases play a role in the mechanism (4 to 6), some scholars believe that AGE can be used as biological characteristics of aging (7). dodder common for Chinese Tradition