Different processed products on the Mustard Seed Mustard determination of alkali thiocyanate.docVIP

 PAGE \* MERGEFORMAT 8 Different processed products on the Mustard Seed Mustard determination of alkali thiocyanate [Abstract] Objective To establish the Mustard Seed Mustard base of different processed products of thiocyanate determination. Methods RP-HPLC method using column was Hypersil BDS phenyl column (5 μm, 250 mm × 4.6 mm), acetonitrile - 0.08 mol / L potassium dihydrogen phosphate solution (15:85) as mobile phase, detection wavelength 240 nm, flow rate 1.0 mL / min. Results sinapine thiocyanate sample volume range of 0.8 ~ 4.0 μg in a good linear relationship , r = 0.999 7, the average recovery was 100.98% (RSD = 3.51%). Conclusion The method is simple, accurate, reproducible, and can be used for Mustard Seed quality control of processed products. [Keywords:] Mustard Seed, sinapine thiocyanate, high performance liquid chromatography Abstract: Objective To develop an HPLC method for the assay of sinapine thiocyanate in Semen Sinapis Albae of different processed products. Methods It was assayed by RP-HPLC. The column was Hypersil BDS Phenyl (5 μm, 250 mm × 4.6 mm), the mobile phase was acetonitrile-0.08 mol / L KH2PO4 (15:85). The UV detection wavelength was 240 nm. The flow rate was 1.0 mL / min. Results Good linearity of sinapine thiocyanate was showed within the range of 0.8 ~ 4.0 μg ( r = 0.999 7). The average recovery was 100.98% (RSD = 3.51%). Conclusion The method is simple, accurate and good reproducible. It can be used for quality control of Semen Sinapis Albae of different processed products. Keywords:: Seman Sinapis Albae, sinapine thiocyanate, HPLC Mustard Seed for the cruciferous 1 year old or more years old is a herb mustard Sinapis alba L. White mustard seeds, spicy, warm, the lung, stomach, with the benefit gas Phlegm, warm in the cold, analgesic Tongluo role, the main component of Mustard Seed glycosides (Sinalbin), myrosinase (Myrosin), sinapine (Sinapine) [1], and mostly sinapine sinapine the form of thiocyanate. Our e