大孔树脂纯化银杏叶黄酮的研究.pdf

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.12 大孔树脂纯化银杏叶黄酮的研究 吴海霞 1， 2，吴彩娥 1，李婷婷 1，范龚健 1，应瑞峰 1 （1.南京林业大学森林资源与环境学院，江苏南京 210037）（ 2.运城学院生命科学系，山西运城 044000） 摘要：以脱脂银杏叶粉为原料，采用 70%乙醇浸提法提取银杏叶黄酮，研究大孔树脂纯化银杏叶黄酮的工艺条件。以吸附率和 解吸率为指标，考察了 AB-8、D101、HPD-100 3 种大孔树脂对银杏叶黄酮的吸附解吸性能，筛选出适合银杏叶黄酮分离纯化的树脂 为 AB-8 型大孔树脂。结合静态与动态吸附解吸试验，得出 AB-8 大孔树脂分离纯化银杏叶黄酮的最佳工艺：将银杏叶黄酮提取原液 稀释 1.5 倍（浓度为 0.94 mg/mL）、调 pH 至 4.85 作为上样液，以 1.5 BV/h 的流速上样吸附，上样量 200 mL，之后采用 pH 4.95 的 80% 乙醇作为洗脱剂，以 2~2.5 BV/h 的流速进行洗脱，洗脱剂用量约 50 mL。在此纯化条件下所得银杏叶黄酮含量为 26.16%，较纯化前 提高了 3.2 倍。该纯化工艺条件科学合理，可有效用于银杏叶黄酮的分离富集，提高银杏叶提取物中的黄酮含量。 关键词：银杏叶；黄酮；大孔树脂；纯化 文章篇号：1673-9078(2013)12-2964-2969 Purification of Flavones from Gingko biloba Leaves by Macroporous Resin WU Hai-xia1,2, WU Cai-e1, LI Ting-ting1, FAN Gong-jian1, YING Rui-feng1 (1.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China) (2.Department of Life Science, Yuncheng University, Yuncheng 044000, China) Abstract: The flavones was extracted with 70% ethanol from degreased powder of Ginkgo biloba leaves (GBLs), and technical conditions of macroporous resins for the purification of flavones from GBLs were studied. The performances of adsorption-desorption of three resins AB-8, D101 and HPD-100 were investigated by using adsorption and desorption rate as indexes. And the AB-8 was considered to be the optimum resin to purify the flavones of GBLs due to its highest adsorption-desorption properties. Combination of static and dynamic experiments o