Effect of simvastatin on human chronic myelogenous leukemia cells K562 gene expression.docVIP

 PAGE \* MERGEFORMAT 14 Effect of simvastatin on human chronic myelogenous leukemia cells K562 gene expression Of: Huang Wenfang, Yang Long, Chuan Liang Min, Liu Hua, who Yali, Zhou Dingan, Xu Guoqiang, Liu [Abstract] Objective To study using gene chip technology simvastatin on gene expression of K562 cells, to explore its mechanism. Methods simvastatin 48h K562 cells, the extracted total cellular RNA. Purification of mRNA and then reverse the cDNA, the cDNA in vitro transcription of synthetic cRNA, with the random primer reverse transcription cDNA, using Klenow enzyme labeled Cy3, Cy5, and the custom includes human whole genome DNA microarray hybridization, scanning the chip fluorescence signal, using GenePix Pro 4.0 image analysis software on the chip image analysis. quantitative RT PCR validation of microarray results of two differentially expressed genes. microarray results detected a total of 176 significant changes in gene expression, including 16 unknown genes expression. 151 upregulated genes, 25 genes down. According to the different gene function, we initially divided into apoptosis, signal transduction, cell cycle-related and other categories. quantitative RT PCR validation of gene expression microarray Trends and consistent results. Conclusion K562 cells after simvastatin can cause a variety of changes in gene expression, which is to explore its mechanism provides a new basis. [Keywords:] K562 cells, simvastatin, microarray, chronic myelogenous leukemia ABSTRACT: Objective To investigate the effect of simvastatin on gene expression profiles of K562 cells and to elucidate its mechanism. Methods The total RNA of K562 cells treated with or without simvastatin for 48h was extracted and purified, followed by cDNA synthesis, then in vitro transcription reaction was subjected to synthesizing cRNA, which was transcripted into cDNA using random primers. cDNA labelled Cy3 and Cy5 by Klenow enzyme was hybridized with genechip, then the chip was scanne